RustEffectorV1, Main, Exploration, bibRecord, 000042

Suppression or Activation of Immune Responses by Predicted Secreted Proteins of the Soybean Rust Pathogen Phakopsora pachyrhizi.

Identifieur interne : 000042 ( Main/Exploration ); précédent : 000041; suivant : 000043

Suppression or Activation of Immune Responses by Predicted Secreted Proteins of the Soybean Rust Pathogen Phakopsora pachyrhizi.

Auteurs : Mingsheng Qi [États-Unis] ; James P. Grayczyk [États-Unis] ; Janina M. Seitz [Allemagne] ; Youngsill Lee [Corée du Sud] ; Tobias I. Link [Allemagne] ; Doil Choi [Corée du Sud] ; Kerry F. Pedley [États-Unis] ; Ralf T. Voegele [Allemagne] ; Thomas J. Baum [États-Unis] ; Steven A. Whitham [États-Unis]

Source :

Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2018.

RBID : pubmed:29144203

Descripteurs français

KwdFr :
- Capsicum (microbiologie), Clonage moléculaire (MeSH), Fractions subcellulaires (métabolisme), Mort cellulaire (MeSH), Phakopsora pachyrhizi (métabolisme), Protéine Bax (métabolisme), Protéines fongiques (métabolisme), Saccharomyces cerevisiae (métabolisme), Soja (immunologie), Soja (microbiologie), Systèmes bactériens de sécrétion (MeSH), Tabac (génétique).
MESH :
- génétique : Tabac.
- immunologie : Soja.
- microbiologie : Capsicum, Soja.
- métabolisme : Fractions subcellulaires, Phakopsora pachyrhizi, Protéine Bax, Protéines fongiques, Saccharomyces cerevisiae.
- Clonage moléculaire, Mort cellulaire, Systèmes bactériens de sécrétion.

English descriptors

KwdEn :
- Bacterial Secretion Systems (MeSH), Capsicum (microbiology), Cell Death (MeSH), Cloning, Molecular (MeSH), Fungal Proteins (metabolism), Phakopsora pachyrhizi (metabolism), Saccharomyces cerevisiae (metabolism), Soybeans (immunology), Soybeans (microbiology), Subcellular Fractions (metabolism), Tobacco (genetics), bcl-2-Associated X Protein (metabolism).
MESH :
- chemical , metabolism : Fungal Proteins, bcl-2-Associated X Protein.
- chemical : Bacterial Secretion Systems.
- genetics : Tobacco.
- immunology : Soybeans.
- metabolism : Phakopsora pachyrhizi, Saccharomyces cerevisiae, Subcellular Fractions.
- microbiology : Capsicum, Soybeans.
- Cell Death, Cloning, Molecular.

Abstract

Rust fungi, such as the soybean rust pathogen Phakopsora pachyrhizi, are major threats to crop production. They form specialized haustoria that are hyphal structures intimately associated with host-plant cell membranes. These haustoria have roles in acquiring nutrients and secreting effector proteins that manipulate host immune systems. Functional characterization of effector proteins of rust fungi is important for understanding mechanisms that underlie their virulence and pathogenicity. Hundreds of candidate effector proteins have been predicted for rust pathogens, but it is not clear how to prioritize these effector candidates for further characterization. There is a need for high-throughput approaches for screening effector candidates to obtain experimental evidence for effector-like functions, such as the manipulation of host immune systems. We have focused on identifying effector candidates with immune-related functions in the soybean rust fungus P. pachyrhizi. To facilitate the screening of many P. pachyrhizi effector candidates (named PpECs), we used heterologous expression systems, including the bacterial type III secretion system, Agrobacterium infiltration, a plant virus, and a yeast strain, to establish an experimental pipeline for identifying PpECs with immune-related functions and establishing their subcellular localizations. Several PpECs were identified that could suppress or activate immune responses in nonhost Nicotiana benthamiana, N. tabacum, Arabidopsis, tomato, or pepper plants.

DOI: 10.1094/MPMI-07-17-0173-FI
PubMed: 29144203

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000052
to stream Main, to step Curation: 000052

Le document en format XML

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<term>Cell Death (MeSH)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>Fungal Proteins (metabolism)</term>
<term>Phakopsora pachyrhizi (metabolism)</term>
<term>Saccharomyces cerevisiae (metabolism)</term>
<term>Soybeans (immunology)</term>
<term>Soybeans (microbiology)</term>
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<term>Tobacco (genetics)</term>
<term>bcl-2-Associated X Protein (metabolism)</term>
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<term>Clonage moléculaire (MeSH)</term>
<term>Fractions subcellulaires (métabolisme)</term>
<term>Mort cellulaire (MeSH)</term>
<term>Phakopsora pachyrhizi (métabolisme)</term>
<term>Protéine Bax (métabolisme)</term>
<term>Protéines fongiques (métabolisme)</term>
<term>Saccharomyces cerevisiae (métabolisme)</term>
<term>Soja (immunologie)</term>
<term>Soja (microbiologie)</term>
<term>Systèmes bactériens de sécrétion (MeSH)</term>
<term>Tabac (génétique)</term>
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<term>bcl-2-Associated X Protein</term>
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<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Bacterial Secretion Systems</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Tobacco</term>
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<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Soybeans</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phakopsora pachyrhizi</term>
<term>Saccharomyces cerevisiae</term>
<term>Subcellular Fractions</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Capsicum</term>
<term>Soja</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Capsicum</term>
<term>Soybeans</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Fractions subcellulaires</term>
<term>Phakopsora pachyrhizi</term>
<term>Protéine Bax</term>
<term>Protéines fongiques</term>
<term>Saccharomyces cerevisiae</term>
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<term>Cloning, Molecular</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term>
<term>Mort cellulaire</term>
<term>Systèmes bactériens de sécrétion</term>
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<front><div type="abstract" xml:lang="en">Rust fungi, such as the soybean rust pathogen Phakopsora pachyrhizi, are major threats to crop production. They form specialized haustoria that are hyphal structures intimately associated with host-plant cell membranes. These haustoria have roles in acquiring nutrients and secreting effector proteins that manipulate host immune systems. Functional characterization of effector proteins of rust fungi is important for understanding mechanisms that underlie their virulence and pathogenicity. Hundreds of candidate effector proteins have been predicted for rust pathogens, but it is not clear how to prioritize these effector candidates for further characterization. There is a need for high-throughput approaches for screening effector candidates to obtain experimental evidence for effector-like functions, such as the manipulation of host immune systems. We have focused on identifying effector candidates with immune-related functions in the soybean rust fungus P. pachyrhizi. To facilitate the screening of many P. pachyrhizi effector candidates (named PpECs), we used heterologous expression systems, including the bacterial type III secretion system, Agrobacterium infiltration, a plant virus, and a yeast strain, to establish an experimental pipeline for identifying PpECs with immune-related functions and establishing their subcellular localizations. Several PpECs were identified that could suppress or activate immune responses in nonhost Nicotiana benthamiana, N. tabacum, Arabidopsis, tomato, or pepper plants.</div>
</front>
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<DateCompleted><Year>2018</Year>
<Month>06</Month>
<Day>12</Day>
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<DateRevised><Year>2018</Year>
<Month>12</Month>
<Day>02</Day>
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<Month>01</Month>
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<Title>Molecular plant-microbe interactions : MPMI</Title>
<ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation>
</Journal>
<ArticleTitle>Suppression or Activation of Immune Responses by Predicted Secreted Proteins of the Soybean Rust Pathogen Phakopsora pachyrhizi.</ArticleTitle>
<Pagination><MedlinePgn>163-174</MedlinePgn>
</Pagination>
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<Abstract><AbstractText>Rust fungi, such as the soybean rust pathogen Phakopsora pachyrhizi, are major threats to crop production. They form specialized haustoria that are hyphal structures intimately associated with host-plant cell membranes. These haustoria have roles in acquiring nutrients and secreting effector proteins that manipulate host immune systems. Functional characterization of effector proteins of rust fungi is important for understanding mechanisms that underlie their virulence and pathogenicity. Hundreds of candidate effector proteins have been predicted for rust pathogens, but it is not clear how to prioritize these effector candidates for further characterization. There is a need for high-throughput approaches for screening effector candidates to obtain experimental evidence for effector-like functions, such as the manipulation of host immune systems. We have focused on identifying effector candidates with immune-related functions in the soybean rust fungus P. pachyrhizi. To facilitate the screening of many P. pachyrhizi effector candidates (named PpECs), we used heterologous expression systems, including the bacterial type III secretion system, Agrobacterium infiltration, a plant virus, and a yeast strain, to establish an experimental pipeline for identifying PpECs with immune-related functions and establishing their subcellular localizations. Several PpECs were identified that could suppress or activate immune responses in nonhost Nicotiana benthamiana, N. tabacum, Arabidopsis, tomato, or pepper plants.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Qi</LastName>
<ForeName>Mingsheng</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>1 Department of Plant Pathology and Microbiology, Iowa State University, Ames 50011, U.S.A.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Grayczyk</LastName>
<ForeName>James P</ForeName>
<Initials>JP</Initials>
<AffiliationInfo><Affiliation>1 Department of Plant Pathology and Microbiology, Iowa State University, Ames 50011, U.S.A.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Seitz</LastName>
<ForeName>Janina M</ForeName>
<Initials>JM</Initials>
<AffiliationInfo><Affiliation>2 Institut für Phytomedizin, Universität Hohenheim, Otto-Sander-Straße 5, 70599 Stuttgart, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lee</LastName>
<ForeName>Youngsill</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>3 Department of Plant Science, Plant Genomics and Breeding Institute, Seoul National University, Seoul 151-921, Korea; and.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Link</LastName>
<ForeName>Tobias I</ForeName>
<Initials>TI</Initials>
<AffiliationInfo><Affiliation>2 Institut für Phytomedizin, Universität Hohenheim, Otto-Sander-Straße 5, 70599 Stuttgart, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Choi</LastName>
<ForeName>Doil</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>3 Department of Plant Science, Plant Genomics and Breeding Institute, Seoul National University, Seoul 151-921, Korea; and.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Pedley</LastName>
<ForeName>Kerry F</ForeName>
<Initials>KF</Initials>
<AffiliationInfo><Affiliation>4 Foreign Disease-Weed Science Research Unit, United States Department of Agriculture-Agricultural Research Service, Ft. Detrick, MD 21702, U.S.A.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Voegele</LastName>
<ForeName>Ralf T</ForeName>
<Initials>RT</Initials>
<AffiliationInfo><Affiliation>2 Institut für Phytomedizin, Universität Hohenheim, Otto-Sander-Straße 5, 70599 Stuttgart, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Baum</LastName>
<ForeName>Thomas J</ForeName>
<Initials>TJ</Initials>
<AffiliationInfo><Affiliation>1 Department of Plant Pathology and Microbiology, Iowa State University, Ames 50011, U.S.A.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Whitham</LastName>
<ForeName>Steven A</ForeName>
<Initials>SA</Initials>
<AffiliationInfo><Affiliation>1 Department of Plant Pathology and Microbiology, Iowa State University, Ames 50011, U.S.A.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>1551452</GrantID>
<Agency>National Science Foundation</Agency>
<Country>International</Country>
</Grant>
<Grant><GrantID>0820642</GrantID>
<Agency>National Science Foundation</Agency>
<Country>International</Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
<PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2017</Year>
<Month>11</Month>
<Day>16</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Mol Plant Microbe Interact</MedlineTA>
<NlmUniqueID>9107902</NlmUniqueID>
<ISSNLinking>0894-0282</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D058947">Bacterial Secretion Systems</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D051028">bcl-2-Associated X Protein</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D058947" MajorTopicYN="N">Bacterial Secretion Systems</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002212" MajorTopicYN="N">Capsicum</DescriptorName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016923" MajorTopicYN="N">Cell Death</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000067563" MajorTopicYN="N">Phakopsora pachyrhizi</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013025" MajorTopicYN="N">Soybeans</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013347" MajorTopicYN="N">Subcellular Fractions</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051028" MajorTopicYN="N">bcl-2-Associated X Protein</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2017</Year>
<Month>11</Month>
<Day>17</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2018</Year>
<Month>6</Month>
<Day>13</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2017</Year>
<Month>11</Month>
<Day>17</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">29144203</ArticleId>
<ArticleId IdType="doi">10.1094/MPMI-07-17-0173-FI</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Allemagne</li>
<li>Corée du Sud</li>
<li>États-Unis</li>
</country>
<region><li>Bade-Wurtemberg</li>
<li>District de Stuttgart</li>
<li>Iowa</li>
<li>Maryland</li>
</region>
<settlement><li>Ames (Iowa)</li>
<li>Stuttgart</li>
<li>Séoul</li>
</settlement>
<orgName><li>Université d'État de l'Iowa</li>
<li>Université nationale de Séoul</li>
</orgName>
</list>
<tree><country name="États-Unis"><region name="Iowa"><name sortKey="Qi, Mingsheng" sort="Qi, Mingsheng" uniqKey="Qi M" first="Mingsheng" last="Qi">Mingsheng Qi</name>
</region>
<name sortKey="Baum, Thomas J" sort="Baum, Thomas J" uniqKey="Baum T" first="Thomas J" last="Baum">Thomas J. Baum</name>
<name sortKey="Grayczyk, James P" sort="Grayczyk, James P" uniqKey="Grayczyk J" first="James P" last="Grayczyk">James P. Grayczyk</name>
<name sortKey="Pedley, Kerry F" sort="Pedley, Kerry F" uniqKey="Pedley K" first="Kerry F" last="Pedley">Kerry F. Pedley</name>
<name sortKey="Whitham, Steven A" sort="Whitham, Steven A" uniqKey="Whitham S" first="Steven A" last="Whitham">Steven A. Whitham</name>
</country>
<country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Seitz, Janina M" sort="Seitz, Janina M" uniqKey="Seitz J" first="Janina M" last="Seitz">Janina M. Seitz</name>
</region>
<name sortKey="Link, Tobias I" sort="Link, Tobias I" uniqKey="Link T" first="Tobias I" last="Link">Tobias I. Link</name>
<name sortKey="Voegele, Ralf T" sort="Voegele, Ralf T" uniqKey="Voegele R" first="Ralf T" last="Voegele">Ralf T. Voegele</name>
</country>
<country name="Corée du Sud"><noRegion><name sortKey="Lee, Youngsill" sort="Lee, Youngsill" uniqKey="Lee Y" first="Youngsill" last="Lee">Youngsill Lee</name>
</noRegion>
<name sortKey="Choi, Doil" sort="Choi, Doil" uniqKey="Choi D" first="Doil" last="Choi">Doil Choi</name>
</country>
</tree>
</affiliations>
</record>

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   |texte=   Suppression or Activation of Immune Responses by Predicted Secreted Proteins of the Soybean Rust Pathogen Phakopsora pachyrhizi.
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	Serveur d'exploration sur les effecteurs de la rouille
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Suppression or Activation of Immune Responses by Predicted Secreted Proteins of the Soybean Rust Pathogen Phakopsora pachyrhizi.

Suppression or Activation of Immune Responses by Predicted Secreted Proteins of the Soybean Rust Pathogen Phakopsora pachyrhizi.

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